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Thermo Fisher
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Thermo Fisher
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Elabscience Biotechnology
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Tanon Science & Technology
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Santa Cruz Biotechnology
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Novus Biologicals
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Image Search Results
Journal: The Journal of Cell Biology
Article Title: An electrostatic switch displaces phosphatidylinositol phosphate kinases from the membrane during phagocytosis
doi: 10.1083/jcb.200909025
Figure Lengend Snippet: Sustained production of PI4,5P 2 inhibits phagocytosis. (a) Schematic of the experimental protocol. The addition of rapamycin induces the heterodimerization of the YFP-FKBP-5K construct with a plasma membrane–targeted form of FRB. (b) Distribution of YFP-FKBP-5K and RFP-PH–PLC-δ before (−Rap) and 5 min after the addition of 10 µM rapamycin (+Rap). Arrowhead identifies sealed and internalized phagosomes, and the arrow highlights forming phagosomes. (c) Internalization and adherence of beads to macrophages was quantified in cells expressing YFP or YFP-FKBP-5K and plasma membrane–targeted FRB with or without the addition of rapamycin. Data are means ± SEM of at least 500 beads per condition.
Article Snippet: To construct the YFP-FKBP-5K plasmid used in the rapamycin experiments, the entire PIP5K-γ87 open reading frame was cloned into the multiple cloning site of the
Techniques: Construct, Expressing
Journal: Pharmaceutics
Article Title: Retinal Protection of New Nutraceutical Formulation
doi: 10.3390/pharmaceutics17010073
Figure Lengend Snippet: Immunostaining of RNA-binding protein and mRNA Processing Factor-positive (RBPMS+) cells for the analysis of retinal ganglion cell (RGC) density in flat-mount retinas. ( a ) RBPMS+ RGCs in central ( A , B ) and peripheral areas ( C , D ) of control (CTRL) and Epicolin formulation-treated mice. Quantification was carried out on the whole retina ( b ) and on both central and peripheral areas of the retina ( c ). Scale bar corresponds to 50 μm. The data were plotted as means ± SEMs (n = 12 retinas per group). * p ≤ 0.05 vs. CTRL; ° p ≤ 0.05 vs. CTRL c; † p ≤ 0.05 vs. CTRL p. Unpaired t -test. CTRL central (CTRL c), CTRL periphery (CTRL p), Epicolin central (Epicolin c), and Epicolin periphery (Epicolin p).
Article Snippet: Primary antibody for RNA binding protein,
Techniques: Immunostaining, RNA Binding Assay, Control, Formulation